Performance evaluation according to CTS (Common Technical Specifications) yielded the following results:
Place one drop of isotonic saline solution on each reaction field and auto-control field.
Add one drop of the recipient’s blood to each field in the upper panel of the card and one drop of donor blood to each field in the lower panel of the card.
Stir each field with an applicator stick for approx. 30 seconds. The reagents must dissolve completely.
Gently rock the card back and forth for approx. 30 to 60 seconds, then check each field for agglutination.
Dry the reaction mixtures and cover with self-adhesive film before filling the card.